# The 30 largest companies with in-house R&D in Prague

Tamtam tracks 40 companies with in-house R&D headquartered in Prague. This list ranks the 30 largest by number of employees on LinkedIn, led by Zentiva, Unicorn and Czech Technical University in Prague. Their median headcount is 630. Half of them were founded in 1990 or later.

These companies with in-house R&D in Prague represent a mix of sectors, including higher education, IT services, and manufacturing. Many are large, well-established organizations, some founded centuries ago. Zentiva is a pan-European pharmaceutical company, Unicorn provides IT solutions, Charles University is a prestigious academic institution, and Seznam.cz is a leading web portal.

A notable pattern is the strong presence of higher education institutions, with Charles University, Czech Technical University in Prague, Czech University of Life Sciences Prague, and University of Chemistry and Technology in Prague all featuring prominently. Additionally, several companies are involved in healthcare, such as Zentiva, Všeobecná fakultní nemocnice v Praze, and BTL Medical & Healthcare Technologies, indicating a focus on health and scientific research.

🇨🇿 Prague · 30 companies · Updated Nov 11, 2026

| # | Company | Industry | Headquarters | Employees | Founded |
|---|---|---|---|---|---|
| 1 | [Zentiva](https://www.tamtam.ai/c/zentiva) (zentiva.com)<br>A Pan-European pharmaceutical company developing, manufacturing, and providing affordable medicines in key therapeutic areas with a focus on growth and supply security | Pharmaceutical Manufacturing | 🇨🇿 Prague | 3,665 | 1488 |
| 2 | [Unicorn](https://www.tamtam.ai/c/unicorn) (unicorn.com)<br>A European IT company providing high value solutions for various industries, offering internet services for small businesses and promoting online education | IT Services and IT Consulting | 🇨🇿 Prague | 2,105 | 1990 |
| 3 | [Czech Technical University in Prague](https://www.tamtam.ai/c/czech-technical-university-in-prague) (cvut.cz)<br>Leading Central European technical institution providing engineering, architecture, and business education through eight faculties, specialized research laboratories, and diverse degree programs for students. | Higher Education | 🇨🇿 Prague | 1,948 | 1707 |
| 4 | [Seznam.cz](https://www.tamtam.ai/c/seznam-cz) (o-seznam.cz)<br>Leading web portal and search engine in the Czech Republic, founded in 1996 Achieved 3 billion CZK revenue in 2014 and continues to grow | Technology, Information and Internet | 🇨🇿 Prague | 1,831 | 1996 |
| 5 | [Charles University](https://www.tamtam.ai/c/charles-university-prague) (cuni.cz)<br>A prestigious university in Prague, founded in 1348, offering a wide range of academic programs with a strong focus on Natural Sciences | Higher Education | 🇨🇿 Prague | 1,798 |  |
| 6 | [České dráhy, a.s.](https://www.tamtam.ai/c/ceske-drahy-a-s) (ceskedrahy.cz)<br>The largest railway carrier in the Czech Republic, providing passenger and freight services with 21,000 employees and a focus on modernizing the country's railway system | Rail Transportation | 🇨🇿 Prague | 1,585 |  |
| 7 | [FF Group](https://www.tamtam.ai/c/ff-group) (ff-group.ai)<br>An innovative data solution provider specializing in Edge and data solutions for road traffic and parking vendors, offering AI intelligence platform for informed decisions | Software Development | 🇨🇿 Prague | 1,386 | 2005 |
| 8 | [Metrostav a.s.](https://www.tamtam.ai/c/metrostav-a-s) (metrostav.cz)<br>A leading construction firm in the Czech Republic, part of a group working on projects domestically and internationally, with a strong reputation and significant revenues | Construction | 🇨🇿 Prague | 1,154 | 1971 |
| 9 | [Czech University of Life Sciences Prague](https://www.tamtam.ai/c/czech-university-of-life-sciences-prague) (czu.cz)<br>A top Prague university with over 21,000 students focusing on economics, management, ecology, technology, agriculture, forestry, and natural sciences | Higher Education | 🇨🇿 Prague | 1,095 | 1906 |
| 10 | [Všeobecná fakultní nemocnice v Praze](https://www.tamtam.ai/c/vseobecna-fakultni-nemocnice-v-praze) (vfn.cz)<br>A leading university hospital in Prague providing a wide range of medical services, innovative treatments, and high-tech medicine at a world-class level | Hospitals and Health Care | 🇨🇿 Prague | 928 | 1790 |
| 11 | [University of Chemistry and Technology in Prague (UCT Prague)](https://www.tamtam.ai/c/university-of-chemistry-and-technology-in-prague-uct-prague) (vscht.cz)<br>Leading educational institution in Central Europe with almost 200 years of tradition, offering advanced technologies, international student exchanges, and prestigious career opportunities | Higher Education | 🇨🇿 Prague | 924 | 1952 |
| 12 | [Make](https://www.tamtam.ai/c/make) (make.com)<br>It is a leading automation platform that allows users to design and build custom solutions for tasks, workflows, and applications. | Software Development | 🇨🇿 Prague | 707 |  |
| 13 | [Fakultní nemocnice Bulovka](https://www.tamtam.ai/c/fakultni-nemocnice-bulovka) (bulovka.cz)<br>A leading Prague hospital providing specialized medical care, with 25 departments, 7 clinics, 2 institutes, and 12 outpatient clinics Includes extensive services and facilities | Hospitals and Health Care | 🇨🇿 Prague | 638 | 1931 |
| 14 | [ComAp Group](https://www.tamtam.ai/c/comap-group) (comap-control.com)<br>Provider of smart power control solutions and services for independent and backup power generation markets, with a focus on flexibility, scalability, and sustainability | Appliances, Electrical, and Electronics Manufacturing | 🇨🇿 Prague | 633 | 1991 |
| 15 | [BTL Medical & Healthcare Technologies](https://www.tamtam.ai/c/btl-medical-and-healthcare-technologies) (btl-kariera.cz)<br>Company brings disruptive technologies to healthcare, focusing on cardiology, physical therapy, and medical aesthetics. Develops and produces devices from idea to serial production, distributing globally. | Medical Equipment Manufacturing | 🇨🇿 Prague | 630 | 1993 |
| 16 | [Digiteq Automotive](https://www.tamtam.ai/c/digiteq-automotive) (digiteqautomotive.com)<br>A leading service provider for automotive electrical and electronic systems, supporting development, testing, integration, and mass deployment for Volkswagen Group projects | Software Development | 🇨🇿 Prague | 625 | 2001 |
| 17 | [Institute for Clinical and Experimental Medicine (IKEM)](https://www.tamtam.ai/c/institute-for-clinical-and-experimental-medicine-ikem) (ikem.cz)<br>A leading clinical and research center in Czech Republic specializing in cardiovascular diseases, organ transplantation, diabetology, and metabolic disorders with extensive facilities and staff | Hospitals and Health Care | 🇨🇿 Prague | 624 | 1971 |
| 18 | [PRO.MED.CS](https://www.tamtam.ai/c/pro-med-cs) (promedcs.com)<br>A leading Czech pharmaceutical company specializing in developing and producing tablets, capsules, and drugs for gastrointestinal, cardiovascular, and musculoskeletal areas Exporting to over 30 countries | Pharmaceutical Manufacturing | 🇨🇿 Prague | 516 | 1989 |
| 19 | [Czech Academy of Sciences](https://www.tamtam.ai/c/czech-academy-of-sciences) (avcr.cz)<br>A research academy in Czech Republic specializing in natural, technical, social, and humanities sciences, promoting international collaboration and knowledge application in society and industries | Research Services | 🇨🇿 Prague | 491 | 1992 |
| 20 | [Prusa Research](https://www.tamtam.ai/c/prusa-research) (prusa3d.com)<br>A leading manufacturer of 3D printers based in Prague, providing innovative solutions for businesses and individuals worldwide with a focus on growth and quality | Mechanical Or Industrial Engineering | 🇨🇿 Prague | 489 | 2012 |
| 21 | [ICZ Group](https://www.tamtam.ai/c/icz-group) (iczgroup.com)<br>We provide integrated software, networking, and security solutions with a wide range of services in system integration, application development, infrastructure, communication, and security | IT Services and IT Consulting | 🇨🇿 Prague | 477 | 1997 |
| 22 | [Bosch Česká republika](https://www.tamtam.ai/c/bosch-ceska-republika) (bosch.cz)<br>A global company known for innovative technologies and groundbreaking inventions in automotive, tools, and more, with over 8000 employees in the Czech Republic | Machinery Manufacturing | 🇨🇿 Prague | 468 |  |
| 23 | [Faculty of Electrical Engineering, Czech Technical University in Prague](https://www.tamtam.ai/c/faculty-of-electrical-engineering-czech-technical-university-in-prague) (fel.cvut.cz)<br>Leading technical university in the Czech Republic offering education in electrical engineering, telecommunications, automation, informatics, and computer science, with strong research collaborations and industry partnerships | Higher Education | 🇨🇿 Prague | 445 |  |
| 24 | [Finshape](https://www.tamtam.ai/c/finshape) (finshape.com)<br>Finshape provides a digital banking platform with personalization capabilities to help European and MENA banks succeed in the digital world | Financial Services | 🇨🇿 Prague | 432 | 2021 |
| 25 | [Národní památkový ústav](https://www.tamtam.ai/c/narodni-pamatkovy-ustav) (npu.cz)<br>A national institution in charge of the state's cultural heritage preservation, conducting research, providing expert advice, and managing accessible historical landmarks | Government Administration | 🇨🇿 Prague | 418 |  |
| 26 | [Second Foundation](https://www.tamtam.ai/c/second-foundation) (second-foundation.eu)<br>A tech company merging science and economics to develop market-beating trading strategies using mathematical models and advanced technology No clients, only internal innovation | Services for Renewable Energy | 🇨🇿 Prague | 365 |  |
| 27 | [Rohlik Group](https://www.tamtam.ai/c/rohlik-group) (rohlik.group)<br>A leading European online grocery delivery service offering fast delivery of a wide range of quality products, including fresh local food and private label brands | Food and Beverage Services | 🇨🇿 Prague | 356 | 2014 |
| 28 | [VŠCHT Praha - UCT Prague](https://www.tamtam.ai/c/vscht-praha-uct-prague) (vscht.cz)<br>Leading educational institution in Central Europe offering progressive fields of study in chemistry, environmental, food, and biochemical technology, and chemical engineering | Research Services | 🇨🇿 Prague | 355 | 1952 |
| 29 | [SCS Software](https://www.tamtam.ai/c/scs-software) (scssoft.com)<br>A Prague-based game developer known for popular simulation video games, including Euro Truck Simulator 2 and American Truck Simulator | Computer Games | 🇨🇿 Prague | 348 | 1997 |
| 30 | [Draslovka](https://www.tamtam.ai/c/draslovka) (draslovka.com)<br>A Czech family company with a strong market position in the CN-based specialty chemical industry, focused on sustainable products and solutions | Chemical Manufacturing | 🇨🇿 Prague | 341 | 1906 |

## How this list is built

Every company on this list has a Tamtam company profile. The industry is the one the company gives on LinkedIn; categories such as SaaS or fintech are classified by Tamtam's AI from the company's website and LinkedIn page; and the headquarters city must match the company's own address. Companies are ranked by the number of employees on LinkedIn, and the list is refreshed every night. Classifications can be wrong, so treat the list as a starting point rather than an official ranking. Last updated Nov 11, 2026.

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- [SaaS companies in Prague](https://www.tamtam.ai/l/saas-companies-prague) (29)
- [IT Services and IT Consulting companies in Prague](https://www.tamtam.ai/l/it-services-and-it-consulting-companies-prague) (28)
- [Professional services firms in Prague](https://www.tamtam.ai/l/professional-services-firms-prague) (26)
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## The same list elsewhere in Czech Republic

- [Companies with in-house R&D in the Czech Republic](https://www.tamtam.ai/l/r-and-d-companies-czech-republic) (99)

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Source: https://www.tamtam.ai/l/r-and-d-companies-prague