These companies with in-house R&D in Prague represent a mix of sectors, including higher education, IT services, and manufacturing. Many are large, well-established organizations, some founded centuries ago. Zentiva is a pan-European pharmaceutical company, Unicorn provides IT solutions, Charles University is a prestigious academic institution, and Seznam.cz is a leading web portal.
A notable pattern is the strong presence of higher education institutions, with Charles University, Czech Technical University in Prague, Czech University of Life Sciences Prague, and University of Chemistry and Technology in Prague all featuring prominently. Additionally, several companies are involved in healthcare, such as Zentiva, Všeobecná fakultní nemocnice v Praze, and BTL Medical & Healthcare Technologies, indicating a focus on health and scientific research.
| # | Company | Employees | |||
|---|---|---|---|---|---|
| 1 |
Zentivazentiva.com
A Pan-European pharmaceutical company developing, manufacturing, and providing affordable medicines in key therapeutic areas with a focus on growth and supply security |
3,665 | |||
| 2 |
Unicornunicorn.com
A European IT company providing high value solutions for various industries, offering internet services for small businesses and promoting online education |
2,105 | |||
| 3 |
Czech Technical University in Praguecvut.cz
Leading Central European technical institution providing engineering, architecture, and business education through eight faculties, specialized research laboratories, and diverse degree programs for students. |
1,948 | |||
| 4 |
Seznam.czo-seznam.cz
Leading web portal and search engine in the Czech Republic, founded in 1996 Achieved 3 billion CZK revenue in 2014 and continues to grow |
1,831 | |||
| 5 |
Charles Universitycuni.cz
A prestigious university in Prague, founded in 1348, offering a wide range of academic programs with a strong focus on Natural Sciences |
1,798 | |||
| 6 |
České dráhy, a.s.ceskedrahy.cz
The largest railway carrier in the Czech Republic, providing passenger and freight services with 21,000 employees and a focus on modernizing the country's railway system |
1,585 | |||
| 7 |
FF Groupff-group.ai
An innovative data solution provider specializing in Edge and data solutions for road traffic and parking vendors, offering AI intelligence platform for informed decisions |
1,386 | |||
| 8 |
Metrostav a.s.metrostav.cz
A leading construction firm in the Czech Republic, part of a group working on projects domestically and internationally, with a strong reputation and significant revenues |
1,154 | |||
| 9 |
Czech University of Life Sciences Pragueczu.cz
A top Prague university with over 21,000 students focusing on economics, management, ecology, technology, agriculture, forestry, and natural sciences |
1,095 | |||
| 10 |
Všeobecná fakultní nemocnice v Prazevfn.cz
A leading university hospital in Prague providing a wide range of medical services, innovative treatments, and high-tech medicine at a world-class level |
928 | |||
| 11 |
University of Chemistry and Technology in Prague (UCT Prague)vscht.cz
Leading educational institution in Central Europe with almost 200 years of tradition, offering advanced technologies, international student exchanges, and prestigious career opportunities |
924 | |||
| 12 | 707 | ||||
| 13 |
Fakultní nemocnice Bulovkabulovka.cz
A leading Prague hospital providing specialized medical care, with 25 departments, 7 clinics, 2 institutes, and 12 outpatient clinics Includes extensive services and facilities |
638 | |||
| 14 |
ComAp Groupcomap-control.com
Provider of smart power control solutions and services for independent and backup power generation markets, with a focus on flexibility, scalability, and sustainability |
633 | |||
| 15 |
BTL Medical & Healthcare Technologiesbtl-kariera.cz
Company brings disruptive technologies to healthcare, focusing on cardiology, physical therapy, and medical aesthetics. Develops and produces devices from idea to serial production, distributing globally. |
630 | |||
Want more companies than this list?This page shows the largest ones only. Connect to Tamtam to see every company that matches, filter by size, industry or growth, and reach their decision-makers. | |||||
| 16 |
Digiteq Automotivedigiteqautomotive.com
A leading service provider for automotive electrical and electronic systems, supporting development, testing, integration, and mass deployment for Volkswagen Group projects |
625 | |||
| 17 |
Institute for Clinical and Experimental Medicine (IKEM)ikem.cz
A leading clinical and research center in Czech Republic specializing in cardiovascular diseases, organ transplantation, diabetology, and metabolic disorders with extensive facilities and staff |
624 | |||
| 18 |
PRO.MED.CSpromedcs.com
A leading Czech pharmaceutical company specializing in developing and producing tablets, capsules, and drugs for gastrointestinal, cardiovascular, and musculoskeletal areas Exporting to over 30 countries |
516 | |||
| 19 |
Czech Academy of Sciencesavcr.cz
A research academy in Czech Republic specializing in natural, technical, social, and humanities sciences, promoting international collaboration and knowledge application in society and industries |
491 | |||
| 20 |
Prusa Researchprusa3d.com
A leading manufacturer of 3D printers based in Prague, providing innovative solutions for businesses and individuals worldwide with a focus on growth and quality |
489 | |||
| 21 |
ICZ Groupiczgroup.com
We provide integrated software, networking, and security solutions with a wide range of services in system integration, application development, infrastructure, communication, and security |
477 | |||
| 22 |
Bosch Česká republikabosch.cz
A global company known for innovative technologies and groundbreaking inventions in automotive, tools, and more, with over 8000 employees in the Czech Republic |
468 | |||
| 23 |
Faculty of Electrical Engineering, Czech Technical University in Praguefel.cvut.cz
Leading technical university in the Czech Republic offering education in electrical engineering, telecommunications, automation, informatics, and computer science, with strong research collaborations and industry partnerships |
445 | |||
| 24 |
Finshapefinshape.com
Finshape provides a digital banking platform with personalization capabilities to help European and MENA banks succeed in the digital world |
432 | |||
| 25 |
Národní památkový ústavnpu.cz
A national institution in charge of the state's cultural heritage preservation, conducting research, providing expert advice, and managing accessible historical landmarks |
418 | |||
| 26 |
Second Foundationsecond-foundation.eu
A tech company merging science and economics to develop market-beating trading strategies using mathematical models and advanced technology No clients, only internal innovation |
365 | |||
| 27 |
Rohlik Grouprohlik.group
A leading European online grocery delivery service offering fast delivery of a wide range of quality products, including fresh local food and private label brands |
356 | |||
| 28 |
VŠCHT Praha - UCT Praguevscht.cz
Leading educational institution in Central Europe offering progressive fields of study in chemistry, environmental, food, and biochemical technology, and chemical engineering |
355 | |||
| 29 |
SCS Softwarescssoft.com
A Prague-based game developer known for popular simulation video games, including Euro Truck Simulator 2 and American Truck Simulator |
348 | |||
| 30 |
Draslovkadraslovka.com
A Czech family company with a strong market position in the CN-based specialty chemical industry, focused on sustainable products and solutions |
341 | |||
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How this list is built
Every company on this list has a Tamtam company profile. The industry is the one the company gives on LinkedIn; categories such as SaaS or fintech are classified by Tamtam's AI from the company's website and LinkedIn page; and the headquarters city must match the company's own address. Companies are ranked by the number of employees on LinkedIn, and the list is refreshed every night. Classifications can be wrong, so treat the list as a starting point rather than an official ranking. Last updated Nov 11, 2026.
More company lists in Prague
- The largest companies in Prague 427
- Multinational companies in Prague 140
- Financial services firms in Prague 50
- Critical infrastructure operators in Prague 49
- Retail chains in Prague 42
- Companies with a mobile app in Prague 37
- Public sector organisations in Prague 36
- Software Development companies in Prague 34
- Financial Services companies in Prague 33
- Corporate groups and holding companies in Prague 33
- E-commerce companies in Prague 30
- Manufacturers in Prague 30
- SaaS companies in Prague 29
- IT Services and IT Consulting companies in Prague 28
- Professional services firms in Prague 26
- Hardware manufacturers in Prague 25
- IT services companies in Prague 25